DUT Rabbit pAb
Sizes: 20µl, 100µl
Catalogue Numbers: A2901-20, A2901-100
Citations, Manuals and SDS Available upon request.
Background: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Synonyms: BMFDMS; dUTPase; DUT
GeneID: 1854
Swiss-Prot: P33316
Reactivity: Human, Mouse, Rat
Calculated Molecular Weight: 27kDa
Observed Molecular Weight: 24kDa
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Tested Applications: ELISA, WB, IHC-P, IF/ICC
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. | Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:1000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:100 - 1:200
Research Areas: Epigenetics Nuclear Signaling, RNA Binding
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: LO2, Jurkat, HeLa
Cellular Location: Mitochondrion, Nucleus
Category: Polyclonal Antibodies
Citations, Manuals and SDS Available upon request.