EIF4G2/p97 Rabbit pAb
Sizes: 20µl, 100µl
Catalogue Numbers: A2897-20, A2897-100
Citations, Manuals and SDS Available upon request.
Background: Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Synonyms: P97; AAG1; DAP5; NAT1; EIF4G2/p97
GeneID: 1982
Swiss-Prot: P78344
Reactivity: Human, Mouse, Rat
Calculated Molecular Weight: 102kDa
Observed Molecular Weight: 100kDa
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 320-490 of human EIF4G2/p97 (NP_001409.3).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Tested Applications: ELISA, WB, IHC-P, IF/ICC, IP
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. | Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:1000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:50 - 1:200 | IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Research Areas: Epigenetics Nuclear Signaling, RNA Binding, Cell Biology Developmental Biology, Apoptosis
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: HeLa, 293T, Jurkat, LO2, Mouse brain
Cellular Location: Adherens Junction, Cytosol, eukaryotic Translation initiation factor 4F Complex
Category: Polyclonal Antibodies
Citations, Manuals and SDS Available upon request.