Calibration beads for super-resolution microscopy 1 vial of 50 µL of a SpheroRuler suspension in PBS pH 7.4
SpheroRuler is a monodispersed suspension of 1µm diameter spheres coated with 647-fluorophores giving a stable blinking in SMLM microscopy. Their consistent size and geometry make them very practical and reliable standards to assess the accuracy of your x-y or z measurements, 3D reconstruction methods or your image quality. Monodispersed and immobile in solution, they can also be used as rulers, for drift correction or as guides to help localize features on your biological samples.
Size: 50µL (AFA-NAN-1000-647)
Catalogue Number: AFA-NAN-1000-647 (50 µL)
Kit description: 1 vial of 50 µL of a SpheroRuler suspension in PBS pH 7.4.
Allows 10 experiments when using the recommended 5µL volume in 35mm glass-bottom dishes.
Concentration: 7.10exp8 particles per mL.
Stability: up to 7 months when stored at 4°C.
Dye: 647-fluorophore (far-red fluorescence).
Compatible with: dSTORM, SRRF, Airyscan confocal, confocal, SEM
The SpheroRuler family is expanding !
Following the success of SpheroRuler beads, we are thrilled to introduce some brand-new SpheroRulers of various sizes and colours that are now ready to join our catalog:
Additional resources:
Results
One SpheroRuler bead imaged using different microscopy techniques
(A, B). Pictures of SpheroRuler beads acquired in wide-field (A) and SRRF-Stream super-resolution (B) imaging. Scale bar = 5µm
(C, D) Local enlargements of A and B pictures respectively.
Scale bar = 1µm
(E) Fluorescence intensity distributions along the solid lines in C and D.
Credits: Yao Baoli - Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, 20lksehg
SpheroRuler beads as a benchmarking tool to assess the impact of different imaging modes on resolution and image quality
SpheroRuler beads were imaged on a confocal microscope with a 63X oil objective.
Credits: Kseniya Korobchevskaya - Institute of Developmental & Regenerative Medicine, University of Oxford, UK
2D and 3D dSTORM images of a SpheroRuler bead acquired with a Leica GSD system
Credits: Lydia Y Li, Nabi Lab, The University of British Columbia, 2023
Protocol overview in 4 steps:
